Composite
Part:BBa_K4864009
Designed by: Jyothishree V Group: iGEM23_SVCE-CHENNAI (2023-10-10)
pAlx-RBS-Cry2Aa-Linker-GFP
This part consists of a pH-inducible promoter, a Cry2Aa-coding sequence, a Tandem GS linker, and a reporter gene. The pH-inducible promoter, Alx (which stands for Alkaline Xylanase), gets activated in the pH range above 7. In order to confirm the secretion of the Cry2Aa protein at the laboratory level, we added the reporter gene (GFP) in fusion with the laccase gene using a 13 Tandem GS linker.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 772
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 772
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1138
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 772
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 772
Illegal AgeI site found at 567 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1218
Illegal BsaI.rc site found at 312
Illegal BsaI.rc site found at 700
Illegal BsaI.rc site found at 2978
Illegal SapI site found at 879
Illegal SapI site found at 939
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Categories
Parameters
None |